Modified indirect immunofluorescence test for serotyping large numbers of Ureaplasma urealyticum clinical isolates
نویسندگان
چکیده
منابع مشابه
Modified metabolic inhibition test for serotyping strains of Ureaplasma urealyticum (T-strain Mycoplasma).
Antisera prepared against the eight recognized serotypes of Ureaplasma urealyticum were tested against the homologous and heterologous antigens by a modified metabolic inhibition test that used bromothymol blue broth in microtiter plates. The method gives clear endpoint determinations which are usually maintained on continued incubation. Antisera against serotype 8 showed cross-reactions with t...
متن کاملExpanded serotyping scheme for Ureaplasma urealyticum strains isolated from humans.
For the last decade the serological heterogeneity of strains of Ureaplasma urealyticum isolated from humans has been represented by eight serotypes. We have identified two additional serotypes, strains Vancouver and Western. Using a modified metabolic inhibition test and a colony indirect epifluorescence method, we tested antisera to the above 10 types against the 11 members of the independent ...
متن کاملDifferences in biofilm development and antibiotic susceptibility among clinical Ureaplasma urealyticum and Ureaplasma parvum isolates.
OBJECTIVES The aim of this work was to study the ability of clinical isolates of Ureaplasma spp. to form biofilms in vitro and to compare the antibiotic susceptibility of sessile cells and their planktonic counterparts. METHODS A total of nine Ureaplasma spp. isolates recovered from unrelated male patients diagnosed with urethritis or chronic prostatitis and two isolates isolated from the uri...
متن کاملDevelopment of an enzyme-linked immunosorbent assay for serotyping ureaplasma urealyticum strains using monoclonal antibodies.
Ureaplasma urealyticum comprises 14 serotypes. The existing serotyping methods all use polyclonal antibodies. These methods are time-consuming and labor-intensive, and they cannot always be performed on primary isolates; in addition, the results are difficult to interpret. We developed a new enzyme-linked immunosorbent assay (ELISA) method using serotype-specific monoclonal antibodies (MAbs) to...
متن کاملModified real-time PCR for detecting, differentiating, and quantifying Ureaplasma urealyticum and Ureaplasma parvum.
We evaluated a previously described quantitative real-time PCR (qPCR) for quantifying and differentiating Ureaplasma parvum and U. urealyticum. Because of nonspecific reactions with Staphylococcus aureus DNA in the U. parvum PCR, we developed a modified qPCR and designed new primers. These oligonucleotides eradicated cross-reactions, indicating higher specificity. The detection limits of the qP...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1987
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.25.1.191-192.1987